fig3

Figure 3. RT-PCR analysis of mesenchymal, hematopoietic and histocompatibility complex gene expression on eAMCs. GAPDH was used as reference gene. As reported in Table 1, expected product sizes: GAPDH 168 bp; CD34 207 bp; CD29 184 bp; CD44 165 bp; CD166 199 bp; CD105 162 bp; MHC-II 178 bp. Annealing temperatures: GAPDH 60 °C; CD34 56 °C; CD29 63 °C; CD44 63 °C; CD166 55 °C; CD105 56 °C; MHC-II 55 °C. RT-PCR: Reverse transcriptase polymerase chain reaction; eAMCs: equine amniotic mesenchymal cells; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MHC-II: major histocompatibility complex II.