fig5

Figure 5. Validation of the expression and function of model-associated genes. (A-F) The expression of FABP4, GRP, ADH1B, CCL19, CALB2, and MDFIC was a significant prognostic indicator in CRC (log-rank test, two-sided); (G) The expression levels of model-associated genes in the normal cell line (NCM460) and CRC cell lines (HT-29, SW480) were detected by RT-qPCR. Data are presented as mean ± SD from three independent biological replicates. Statistical significance was determined by one-way ANOVA followed by Tukey’s post-hoc test for multiple comparisons. ** indicates P < 0.01, and *** indicates P < 0.001. CRC: Colorectal cancer; RT-qPCR: reverse transcription quantitative polymerase chain reaction; SD: standard deviation; ANOVA: analysis of variance; HR: hazard ratio.